NMR for non-experts; a practical guide for applying NMR methods in studies of aptamer-ligand interactions

METHOD/PROTOCOL

ISSN: 2514-3247
Aptamers (2020), Vol 4, 03-09

Published online: 29 September 2020

Full Text (Churcher ~895kb) | (PubMed Central Record HTML) | (PubMed) | (References)

Zachary R Churcher and Philip E Johnson*

Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, Toronto, Ontario, Canada, M2R 1A1

*Correspondence to: Philip Johnson, Email: pjohnson@yorku.ca, Tel: +1 416 736 2100 x33119

Received: 17 June 2020 | Revised: 23 September 2020 | Accepted: 29 September 2020

© Copyright The Author(s). This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction of this article, provided the original work is appropriately acknowledged, with correct citation details.

ABSTRACT

Nuclear Magnetic Resonance (NMR) Spectroscopy is a powerful technique for studying aptamer structure and function. Important information about ligand binding and how binding affects aptamer structure can be obtained relatively quickly using ¹H NMR spectra focused on the imino resonances. Here we summarise some practical information for preparing aptamer samples for NMR analysis, designing experiments, and interpreting results, with an emphasis on aptamer-small molecule interactions.

KEYWORDS: Aptamers, Nuclear Magnetic Resonance Spectroscopy, folding, small molecule interactions