The minimum aptamer publication standards (MAPS guidelines) for de novo aptamer selection



ISSN: 2514-3247
(2022), Vol 6, 10-18

Published online: 24 May 2022

Full Text (McKeague ~340kb) | (PubMed Central Record HTML) | (PubMed) | (References)

Maureen McKeague1, 2, Victoria Calzada3, Laura Cerchia4, Maria DeRosa5, Jennifer M Heemstra6, Nebojsa Janjic7, Philip E Johnson8, Leon Kraus9, Janice Limson10, Günter Mayer11, 12, Marit Nilsen-Hamilton13, 14, 15, David Porciani16, 17, Tarun Kumar Sharma18, 19,20, Beatrix Suess9, Julian A Tanner21, 22, Sarah Shigdar23, 24*

1Pharmacology and Therapeutics, Faculty of Medicine, McGill University, Montreal, QC H3G 1Y6, Canada

2Department of Chemistry, Faculty of Science, McGill University, Montreal, QC H3A 0B8, Canada

3Área de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay

4Institute of Experimental Endocrinology and Oncology “Gaetano Salvatore”, CNR, Via S. Pansini 5, 80131, Naples, Italy

5Department of Chemistry, 203 Steacie Building, Carleton University, 1125, Colonel By Drive, Ottawa, ON K1S 5B6, Canada

6Department of Chemistry, Emory University, Atlanta, Georgia, United States

7SomaLogic, Inc., 2945 Wilderness Place, Boulder, CO 80301, USA

8Department of Chemistry & Centre for Research on Biomolecular Interactions, York University, 4700 Keele St., Toronto, Ontario, Canada, M3J 1P3

9Department of Biology, TU Darmstadt, 64287 Darmstadt, Germany

10Biotechnology Innovation Centre, Rhodes University, Makhanda, 6140, South Africa

11Chemical Biology & Chemical Genetics, Life and Medical Sciences (LIMES) Institute, University of Bonn, 53121 Bonn, Germany

12Center of Aptamer Research & Development (CARD), University of Bonn, 53121 Bonn, Germany

13Roy J Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA

14Ames Laboratory, US DOE (United States Department of Energy), Ames, IA 50011, USA

15Aptalogic Inc., Ames, IA 50014, USA

16Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, MO 65212, USA

17Bond Life Sciences Center, University of Missouri, Columbia, MO 65201, USA

18Department of Medical Biotechnology, Gujarat Biotechnology University, Gujarat International Finance Tec-City, Gandhinagar, Gujarat 382355, India

19AptaBharat Innovation Pvt Ltd, BBB Bionest Bioincubator, NCR Biotech Science Cluster, 3rd Milestone, Faridabad – Gurgaon Expressway, PO box #04, Faridabad 121001, India

20Translational Research Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 3rd Milestone, Faridabad- Gurgaon Expressway, PO box #04, Faridabad- 121001, India

21School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR 999077, China

22Advanced Biomedical Instrumentation Centre, Hong Kong Science Park, Shatin, New Territories, Hong Kong SAR 999077, China

23School of Medicine, Deakin University, Geelong, VIC 3220, Australia

24Institute for Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University, Geelong, VIC 3220, Australia

*Correspondence to: Sarah Shigdar, Email: (on behalf of the International Society on Aptamers and the Aptamer Consortium)

Received: 16 March 2022 | Revised: 06 May 2022 | Accepted: 18 May 2022

© Copyright The Author(s). This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License ( This license permits non-commercial use, distribution and reproduction of this article, provided the original work is appropriately acknowledged, with correct citation details.


Aptamers were first described in 1990 and since then many aptamers have been reported in the literature for numerous applications in both diagnostics and therapeutics. However, as with most fields, missing or unclear information presented in the publication makes it difficult to replicate some of the work described in the literature. To increase the reproducibility of the data and facilitate academic laboratories and industrial companies to develop reliable aptamer work, essential guidelines should be proposed and followed in any aptamer publication, especially in those that highlight de novo aptamer sequences. Here, we provide suggestions for authors, reviewers, and editors to follow when performing and reporting their aptamer work to ensure that we meet the minimum standards for publication of future aptamer sequences.

KEYWORDS: aptamers, guidelines, in vitro selection, minimum standards, reproducibility, SELEX

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