A guide to using nucleic acid aptamers in cell based assays

TECHNICAL REPORT

OpenAccess

ISSN: 2514-3247
Aptamers 
(2019), Vol 3, in press

Published online: 23 November 2019

Full Text (PDF ~264kb) | (PubMed Central Record HTML) | (PubMed) | (References)

Justin Henri1, Narges Bayat3, 4, Joanna Macdonald1, 2, and Sarah Shigdar1,2,*

1School of Medicine Deakin University, Geelong, Victoria 3128, Australia

2Centre for Molecular and Medical Research, Deakin University, Geelong, Victoria, 3216, Australia

3Leukaemia Biology Program, Children’s Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Australia

4School of Women’s and Children’s Health, Faculty of Medicine, UNSW Sydney, NSW, Australia

*Correspondence to: Sarah Shigdar, Email: Sarah.shigdar@deakin.edu.au, Tel: +613 5227 2846


Received: 23 September 2019 | Revised: 19 November 2019 | Accepted: 22 November 2019


© Copyright The Author(s). This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction of this article, provided the original work is appropriately acknowledged, with correct citation details.


ABSTRACT

Aptamers are single stranded nucleic acid molecules that fold into complex three-dimensional conformations. They have been used for a number of years as replacements for monoclonal antibodies by a core group of researchers. However, now that more and more companies are offering aptamers commercially for research and development applications, there is a need for a ‘how to’ guide for researchers who are interested in using aptamers for their own applications. Within this article, we detail the critical concerns for working with aptamers, from what to dilute them in, concentrations and times to use for incubation steps, as well as a suggested protocol. We hope that this guide will prove useful for researchers who are trialling aptamers for cell based assays, and as a starting point for researchers investigating aptamers for other applications.

Keywords: aptamers, how to, method, protocol, technique

 

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